John 3:1-6 (ANIV)
Jesus teachers Nicodemus
1 Now there was a man of the Pharisees named Nicodemus, a member of the Jewish ruling council. 2 He came to Jesus at night and said, “Rabbi, we know you are a teacher who has come from God. For no-one could perform the miraculous signs you are doing if God were not with him.”
3 In reply Jesus declared, “I tell you the truth, no-one can see the kingdom of God unless he is born again.” 
4 “How can a man be born when he is old?” Nicodemus asked. “Surely he cannot enter a second time into his mother’s womb to be born!”
5 Jesus answered, “I tell you the truth, no-one can enter the kingdom of God unless he is born of water and the Spirit. 6 Flesh gives birth to flesh, but the Spirit  gives birth to spirit. 7 You should not be surprised at my saying, ‘You  must be born again.’
Or, born from above; also in John 3:7.
Or, but spirit.
The Greek is plural.
TIME OF DAY:
Some mysterious lighting from above employed, to avoid making a flat & lifeless cartoon.
Jesus & Nicodemus
The cartoon is to show the debate & conversatioon between Jesus & Nicodemus. This is a friendly interaction, hence the smiles!
I think this cartoon was drawn after the “Jesus & Pharisees” one (John 08), hence Nicodemus looking similar in colouration to those other Pharisees.